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Product Details
Source
Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
Clone
M3HC
Isotype
Human IgG1
Conjugate
Acridine ester
Antibody Type
Recombinant Monoclonal
Reactivity
Human
Immunogen
Human IgM mu Chain
Specificity
This product is a specific antibody specifically reacts with Anti-Human IgM.
Purity
90% as determined by SDS-PAGE.
95% as determined by SEC-MALS.
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH6.3 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) (Cat. No. AHM-S300) is more than 95% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.
Report
Bioactivity-MPCLIA
Immobilized 0.04 μg /Test of Biotinylated SARS-CoV-2 Spike RBD, His,Avitag(Cat. No.SPD-C82E9) to the Streptavidin-Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A006) (10 μg beads/Test), incubated with 100 μL /Test of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgM (AM122) (Cat. No. SPD-M162) at increasing concentration coupled to the Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) (MALS verified) (Cat. No. AHM-S300, 0.04 μg /Test). Detection was performed with sensitivity of 0.488 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
Protocol
The MPCLIA assay shows that Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) (MALS verified) (Cat. No. AHM-S300) is stable at 37°C for 48 hours.
Protocol
The MPCLIA assay shows that Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) (MALS verified) (Cat. No. AHM-S300) is stable after freezing and thawing 3 times.
Protocol
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