HRSV (B) Glycoprotein G Protein, His Tag

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Cat. No. / Size
Price
Qty
GLG-V5144-50ug
$450.00
GLG-V5144-500ug
$2300.00
ETA of in-stock products:2 business days

Product Details

  • Synonym

    Glycoprotein G/G protein (RSV)

  • Source

    HRSV (B) Glycoprotein G Protein, His Tag (GLG-V5144) is expressed from E. coli cells. It contains AA His 67- Ala 299 (Accession # O36633).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    Glycoprotein G/G protein (RSV) Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 27.6 kDa. The protein migrates as 33-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Glycoprotein G/G protein (RSV) SDS-PAGE

    HRSV (B) Glycoprotein G Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     Glycoprotein G/G protein (RSV) ELISA

    Immobilized HRSV (B) Glycoprotein G Protein, His Tag (Cat. No. GLG-V5144) at 1 μg/mL (100 μL/well) can bind Anti-Respiratory Syncytial Virus G Glycoprotein Antibody, Mouse IgG1 with a linear range of 0.2-8 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     Glycoprotein G/G protein (RSV) SPR

    HRSV (B) Glycoprotein G Protein, His Tag (Cat. No. GLG-V5144) immobilized on CM5 Chip can bind Anti-Respiratory Syncytial Virus G Glycoprotein antibody with an affinity constant of 91.9 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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Background

The two major glycoproteins on the surface of the RSV virion, the attachment glycoprotein (G) and the fusion (F) glycoprotein, control the initial phases of infection. The central region of the G protein contains a 13-amino acid highly conserved domain, partially overlapping the cysteine noose domain with 4 cysteines linked 1–4 and 2–3, followed by a highly basic heparin-binding domain (HBD). The HBD is the likely attachment site for heparan sulfate (HS) found on the surface of most cells. A peptide from the G protein HBD (amino acids 184–198) binds efficiently to HEp-2 cells and inhibits RSV infection.

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