Product Details
Synonym
Glycoprotein G/G protein (RSV)
Source
HRSV (B) Glycoprotein G Protein, His Tag (GLG-V5144) is expressed from E. coli cells. It contains AA His 67- Ala 299 (Accession # O36633).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 27.6 kDa. The protein migrates as 33-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE

HRSV (B) Glycoprotein G Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA

Immobilized HRSV (B) Glycoprotein G Protein, His Tag (Cat. No. GLG-V5144) at 1 μg/mL (100 μL/well) can bind Anti-Respiratory Syncytial Virus G Glycoprotein Antibody, Mouse IgG1 with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
Bioactivity-SPR

HRSV (B) Glycoprotein G Protein, His Tag (Cat. No. GLG-V5144) immobilized on CM5 Chip can bind Anti-Respiratory Syncytial Virus G Glycoprotein antibody with an affinity constant of 91.9 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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