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Détails du produit
Synonym
GPRC5D, G-protein coupled receptor family C group 5 member D
Source
Human GPRC5D Full Length Protein (VLP) (GPD-H52P7) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Val 345 (Accession # Q9NZD1-1).
Predicted N-terminus: Asp
Molecular Characterization
Virus-like particles(VLPs) are formed by self-assembly of capsid proteins from viruses. Membrane Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.
The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SEC-HPLC.
Formulation
The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Supplied as 0.2 μm filtered solution in PBS, Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
ACRO Quality Management System
- *The isotype control of empty/mock VLP (Cat. No. VLP-N5213) is sold separately and not included in protein, you can follow this link for product information.
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Affichage des données
SEC-HPLC
The purity of Human GPRC5D Full Length Protein (VLP) (Cat. No. GPD-H52P7) was greater than 95% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Human GPRC5D Full Length Protein (VLP) (Cat. No. GPD-H52P7) at 1 μg/mL (100 μL/well) can bind Anti-GPRC5D Antibody, Human IgG4 with a linear range of 0.2-1 ng/mL (QC tested).
Protocol
Bioactivity-Stability
The accelerated stability based assay shows that Human GPRC5D Full Length Protein (VLP) (Cat. No. GPD-H52P7) is stable at 37°C for 48h.
The freeze-thaw stability based assay shows that Human GPRC5D Full Length Protein (VLP) (Cat. No. GPD-H52P7) is stable after freeze-thaw 3 times.
Identity-DLS
The mean peak Radius of VLP is 55-75 nm with more than 95% intensity as determined by dynamic light scattering (DLS).
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
G-protein coupled receptor family C group 5member D
- Catégorie：
- Nombre de médicaments répertoriés：
1 Détails
- Nombre de médicaments cliniques：
38 Détails
- Étape de développement la plus élevée：
Approved
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