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Product Details
Synonym
PDCD1, PD1, CD279, SLEB2
Source
Biotinylated Feline PD-1 Protein, His,Avitag (PD1-C82E3) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gly 168 (Accession # A9YUA6).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 19.8 kDa. The protein migrates as 30-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Feline PD-1 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Feline PD-1 Protein, His,Avitag (Cat. No. PD1-C82E3) is more than 95% and the molecular weight of this protein is around 27-37 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Biotinylated Feline PD-1 Protein, His,Avitag (Cat. No. PD1-C82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Feline PD-L1, Fc tag with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Feline PD-1 Protein, His,Avitag (Cat. No. PD1-C82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) with a linear range of 0.2-1 ng/mL (Routinely tested).
Protocol
Immobilized Biotinylated Feline PD-1 Protein, His,Avitag (Cat. No. PD1-C82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human PD-L1, Fc Tag (Cat. No. PD1-H5258) with a linear range of 0.2-1 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Programmed cell death protein 1
- Category：
- Approved Drugs：
22 Details
- Drugs in Clinical Trials：
193 Details
- Highest Development Stage：
Approved
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