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Détails du produit
Synonym
PDCD1, PD1, CD279, SLEB2
Source
Human PD-1, Mouse IgG2a Fc Tag (PD1-H5255) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 42.5 kDa. The protein migrates as 55-66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human PD-1, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human PD-1, Mouse IgG2a Fc Tag (Cat. No. PD1-H5255) is more than 95% and the molecular weight of this protein is around 95-130 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at 10 μg/mL (100 μL/well) can bind Human PD-1, Mouse IgG2a Fc Tag (Cat. No. PD1-H5255) with a linear range of 4-63 ng/mL (QC tested).
Protocol
Immobilized Human PD-1, Mouse IgG2a Fc Tag (Cat. No. PD1-H5255) at 2 μg/mL (100 μL/well) can bind Human PD-L2, Fc Tag (Cat. No. PD2-H5251) with a linear range of 0.2-4 ng/mL (Routinely tested).
Protocol
Immobilized Human PD-1, Mouse IgG2a Fc Tag (Cat. No. PD1-H5255) at 2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human PD-1, Mouse IgG2a Fc Tag (Cat. No. PD1-H5255) on Protein A Biosensor, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 2.6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human PD-1, Mouse IgG2a Fc Tag (Cat. No. PD1-H5255) on Protein A Biosensor, can bind Human PD-L2, His Tag (Cat. No. PD2-H5220) with an affinity constant of 0.43 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Programmed cell death protein 1
- Catégorie：
- Nombre de médicaments répertoriés：
22 Détails
- Nombre de médicaments cliniques：
193 Détails
- Étape de développement la plus élevée：
Approved
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