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Détails du produit
Synonym
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Source
MABSol® Biotinylated Mouse PD-L1, Fc,Avitag (PD1-M82F5) is expressed from human HEK293 cells. It contains AA Phe 19 - Thr 238 (Accession # NP_068693).
Predicted N-terminus: Phe 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 53.2 kDa. The protein migrates as 65-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Biotinylated Mouse PD-L1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Mouse PD-L1, Fc,Avitag (Cat. No. PD1-M82F5) is more than 95% and the molecular weight of this protein is around 115-150 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Mouse PD-L1, Fc,Avitag (Cat. No. PD1-M82F5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse PD-1 Protein, Mouse IgG2a Fc Tag (Cat. No. PD1-M5257) with a linear range of 5-39 ng/mL (QC tested).
Protocol
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Introduction de fond
Progrès de frontière
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Programmed death-ligand 1
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