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Détails du produit
Application
Synonym
STEAP1, PRSS24, STEAP
Source
Human STEAP1 Full Length Protein, Twin-Strep,His Tag (ST1-H5286) is expressed from human 293 cells (HEK293). It contains AA Glu 2 - Leu 339 (Accession # Q9UHE8-1).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a twin strep tag at the N-terminus and a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 44.7 kDa.
Structure
Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.
Synthesis Process
Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.
Purity
>90% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
- *The isotype control of empty/mock nanodisc (Cat. No. APO-H51H3) is sold separately and not included in protein, you can follow the link for product information.
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Affichage des données
Bioactivity-ELISA
Immobilized Human STEAP1 Full Length Protein, Twin-Strep,His Tag (Cat. No. ST1-H5286) at 5 μg/mL (100 μL/well) on a Nickel Coated plate (Cat. No. SP-19) can bind Vandortuzumab biosimilar with a linear range of 0.005-0.156 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Monoclonal Anti-STEAP1 Antibody, Human IgG1 captured on Protein A Chip can bind Human STEAP1 Full Length Protein, Twin-Strep,His Tag (Cat. No. ST1-H5286) with an affinity constant of 8.04 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-FACS
Non-specificity of Human STEAP1 Protein, Twin-Strep, His Tag (Nanodisc) (Cat. No. ST1-H5286) binding to CD3+T cells present in human PBMC. Human PBMCs were simultaneously stained with FITC-labeled anti-CD3 antibody and Human STEAP1 Protein, Twin-Strep,His Tag (Nanodisc) (5e5 cells were stained with 100 μL of 3 μg/mL of Human STEAP1 Protein, Twin-Strep,His Tag), washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
Protocol
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Introduction de fond
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Progrès du développement médicamenteux- Nom anglais：
Six-transmembrane epithelial antigen of the prostate 1
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